Description
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with anantibody specific to TGF-beta2. Standards or samples are then added tothe appropriate microtiter plate wells with a biotin-conjugatedantibody preparation specific for TGF-beta2 and Avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate well andincubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substratesolution is added to each well. Only those wells that contain TGF-beta2,biotin-conjugated antibody and enzyme-conjugated Avidin will exhibita change in color. The enzyme-substrate reaction is terminated bythe addition of a sulphuric acid solution and the color change ismeasured spectrophotometrically at a wavelength of 450 nm +/- 2 nm.The concentration of TGF-beta2 in the samples is then determined bycomparing the O.D. of the samples to the standard curve